PT  - JOURNAL ARTICLE
AU  - TOSHIHIRO TANAKA
AU  - HIROAKI UCHIDA
TI  - Inhibition of Survivin by Adenovirus Vector Enhanced Paclitaxel-induced Apoptosis in Breast Cancer Cells
AID  - 10.21873/anticanres.12725
DP  - 2018 Jul 01
TA  - Anticancer Research
PG  - 4281--4288
VI  - 38
IP  - 7
4099  - http://ar.iiarjournals.org/content/38/7/4281.short
4100  - http://ar.iiarjournals.org/content/38/7/4281.full
SO  - Anticancer Res2018 Jul 01; 38
AB  - Background/Aim: Survivin expression has been shown to be associated with cancer progression, poor prognosis, and drug resistance. The aim of this study was to examine whether survivin knock-down could enhance paclitaxel-induced apoptosis in breast cancer cells in vitro. Materials and Methods: MCF-7 cells were infected with an siRNA-expressing adenovirus vector against survivin (Adv-siSurv) or Renilla luciferase as a control (Adv-siRL). After treatment with paclitaxel, cells were analyzed by apoptotic, cell cycle and immunoblotting assays. Results: Of cells treated with paclitaxel alone, only 20.2±2.08% showed apoptotic features. An increase in the paclitaxel dose was associated with increased survivin expression. In contrast, Adv-siSurv infection resulted in a marked increase in apoptotic cell death in paclitaxel-treated MCF-7 cells (49.9±7.70%). The percentage of cells in the G2M phase was lower (23.9±1.64%) in Adv-siSurv-infected cells than that of Adv-siRL–treated cells (40.0±2.43%). Adv-siSurv infection reduced survivin, procaspase-9, and procaspase-3 levels in paclitaxel-treated MCF-7 cells. Conclusion: Loss of survivin expression enhanced paclitaxel-induced apoptosis in MCF-7 breast cancer cells in vitro.