PT  - JOURNAL ARTICLE
AU  - ANDREW T. MERAM
AU  - JIE CHEN
AU  - STAVAN PATEL
AU  - DONGSOO D. KIM
AU  - BRETT SHIRLEY
AU  - PAUL COVELLO
AU  - DOMENICO COPPOLA
AU  - ERIC X. WEI
AU  - GHALI GHALI
AU  - CHRISTOPHER G. KEVIL
AU  - RODNEY E. SHACKELFORD
TI  - Hydrogen Sulfide Is Increased in Oral Squamous Cell Carcinoma Compared to Adjacent Benign Oral Mucosae
AID  - 10.21873/anticanres.12668
DP  - 2018 Jul 01
TA  - Anticancer Research
PG  - 3843--3852
VI  - 38
IP  - 7
4099  - http://ar.iiarjournals.org/content/38/7/3843.short
4100  - http://ar.iiarjournals.org/content/38/7/3843.full
SO  - Anticancer Res2018 Jul 01; 38
AB  - Background/Aim: Hydrogen sulfide (H2S) and the enzymes that synthesize it, cystathionine-b-synthase, cystathionine γ-lyase, and 3-mercaptopyruvate, are increased in different human malignancies. Due to its short half-life, H2S concentrations have not been directly measured in a human malignancy. Here we directly measured in vivo H2S levels within oral squamous cell carcinoma (OSCC). Patients and Methods: Punch biopsies of OSCC and benign mucosae from 15 patients were analyzed by HPLC, western blotting, and tissue microarray analyses. Results: H2S concentrations were significantly higher in OSCC compared to adjacent benign oral mucosae. Western blot and tissue microarray studies revealed significantly increased cystathionine-b-synthase, cystathionine γ-lyase, and 3-mercaptopyruvate, phopho-Stat3, mitoNEET, hTERT, and MAPK protein levels in OSCC. Conclusion: H2S concentrations and the enzymes that synthesize it are significantly increased in OSCC. Here, for the first time H2S concentrations within a living human malignancy were measured and compared to adjacent counterpart benign tissue.