TY - JOUR T1 - Hydrogen Sulfide Is Increased in Oral Squamous Cell Carcinoma Compared to Adjacent Benign Oral Mucosae JF - Anticancer Research JO - Anticancer Res SP - 3843 LP - 3852 DO - 10.21873/anticanres.12668 VL - 38 IS - 7 AU - ANDREW T. MERAM AU - JIE CHEN AU - STAVAN PATEL AU - DONGSOO D. KIM AU - BRETT SHIRLEY AU - PAUL COVELLO AU - DOMENICO COPPOLA AU - ERIC X. WEI AU - GHALI GHALI AU - CHRISTOPHER G. KEVIL AU - RODNEY E. SHACKELFORD Y1 - 2018/07/01 UR - http://ar.iiarjournals.org/content/38/7/3843.abstract N2 - Background/Aim: Hydrogen sulfide (H2S) and the enzymes that synthesize it, cystathionine-b-synthase, cystathionine γ-lyase, and 3-mercaptopyruvate, are increased in different human malignancies. Due to its short half-life, H2S concentrations have not been directly measured in a human malignancy. Here we directly measured in vivo H2S levels within oral squamous cell carcinoma (OSCC). Patients and Methods: Punch biopsies of OSCC and benign mucosae from 15 patients were analyzed by HPLC, western blotting, and tissue microarray analyses. Results: H2S concentrations were significantly higher in OSCC compared to adjacent benign oral mucosae. Western blot and tissue microarray studies revealed significantly increased cystathionine-b-synthase, cystathionine γ-lyase, and 3-mercaptopyruvate, phopho-Stat3, mitoNEET, hTERT, and MAPK protein levels in OSCC. Conclusion: H2S concentrations and the enzymes that synthesize it are significantly increased in OSCC. Here, for the first time H2S concentrations within a living human malignancy were measured and compared to adjacent counterpart benign tissue. ER -