RT Journal Article
SR Electronic
T1 Possible Application of Ascites-infiltrating Gamma-delta T Cells for Adoptive Immunotherapy
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 4327
OP 4331
DO 10.21873/anticanres.12732
VO 38
IS 7
A1 ABE, YUKI
A1 KOBAYASHI, HIROHITO
A1 AKIZAWA, YOSHIKA
A1 ISHITANI, KEN
A1 HASHIMOTO, KAZUNORI
A1 MATSUI, HIDEO
YR 2018
UL http://ar.iiarjournals.org/content/38/7/4327.abstract
AB Background/Aim: Malignant ascites contain many tumour-infiltrating lymphocytes. γδ T cells with antitumour activity have attracted attention as effector cells in cancer immunotherapy. Vδ2+ T cells were cultured from peripheral blood mononuclear cells (PBMCs) and ascites-infiltrating lymphocytes (AILs) to compare the differences in response to 2-methyl-3-butenyl-1-pyrophosphate (2M3B1-PP) and zoledronate (Zol) as antigens in vitro. Materials and Methods: To expand Vδ2+ T cells from PBMCs and AILs from 29 patients with cancer, these cells were cultured and subjected to analysis. Results: The proliferation rate of Vδ2+ T cells was higher in both PBMCs and AILs when cultured with Zol than with 2M3B1-PP. Although Vδ2+ T cells show a higher rate of expansion in AILs compared to PBMCs, the number of mixed tumour cells in ascites was decreased when cultured with Zol. Conclusion: Vδ2+ T cells in AILs are cytotoxic to tumour cells in ascites and may be considered in adoptive immunotherapy.