PT  - JOURNAL ARTICLE
AU  - ABE, YUKI
AU  - KOBAYASHI, HIROHITO
AU  - AKIZAWA, YOSHIKA
AU  - ISHITANI, KEN
AU  - HASHIMOTO, KAZUNORI
AU  - MATSUI, HIDEO
TI  - Possible Application of Ascites-infiltrating Gamma-delta T Cells for Adoptive Immunotherapy
AID  - 10.21873/anticanres.12732
DP  - 2018 Jul 01
TA  - Anticancer Research
PG  - 4327--4331
VI  - 38
IP  - 7
4099  - http://ar.iiarjournals.org/content/38/7/4327.short
4100  - http://ar.iiarjournals.org/content/38/7/4327.full
SO  - Anticancer Res2018 Jul 01; 38
AB  - Background/Aim: Malignant ascites contain many tumour-infiltrating lymphocytes. γδ T cells with antitumour activity have attracted attention as effector cells in cancer immunotherapy. Vδ2+ T cells were cultured from peripheral blood mononuclear cells (PBMCs) and ascites-infiltrating lymphocytes (AILs) to compare the differences in response to 2-methyl-3-butenyl-1-pyrophosphate (2M3B1-PP) and zoledronate (Zol) as antigens in vitro. Materials and Methods: To expand Vδ2+ T cells from PBMCs and AILs from 29 patients with cancer, these cells were cultured and subjected to analysis. Results: The proliferation rate of Vδ2+ T cells was higher in both PBMCs and AILs when cultured with Zol than with 2M3B1-PP. Although Vδ2+ T cells show a higher rate of expansion in AILs compared to PBMCs, the number of mixed tumour cells in ascites was decreased when cultured with Zol. Conclusion: Vδ2+ T cells in AILs are cytotoxic to tumour cells in ascites and may be considered in adoptive immunotherapy.