TY - JOUR T1 - Phagocytic Activation of Macrophages with Serum MAF Depends on Engulfment Efficiency and Not Migratory Activity JF - Anticancer Research JO - Anticancer Res SP - 4295 LP - 4298 DO - 10.21873/anticanres.12727 VL - 38 IS - 7 AU - RIHO MASHIBA AU - MAMI ISHIKAWA AU - YU SUMIYA AU - KUMPEI KAWAKATSU AU - NGOC KIET TRAN AU - TAKAHITO NISHIKATA Y1 - 2018/07/01 UR - http://ar.iiarjournals.org/content/38/7/4295.abstract N2 - Background/Aim: Serum-derived macrophage activating factor (serum MAF) is known to increase the phagocytic activity of macrophages and potentially plays a role in activating cancer immunity. In order to reveal the contributing factors for phagocytic activation, the migratory activity and the efficiency of engulfment was analyzed. Materials and Methods: THP-1 macrophages were induced by 12-O-tetradecanoyl-13-acetate (TPA). The migratory activity and efficiency of engulfment were analyzed by time-lapse imaging and suspension assay, respectively. Results: While the distance of migration did not change before and after activation with serum MAF, the efficiency of beads internalisation was significantly increased. Conclusion: Phagocytic activation of serum-MAF-treated macrophages was caused by increasing the efficiency of engulfment. This study contributes to the knowledge about the activation of the immune system through phagocytic activation of macrophages. ER -