RT Journal Article
SR Electronic
T1 Phagocytic Activation of Macrophages with Serum MAF Depends on Engulfment Efficiency and Not Migratory Activity
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 4295
OP 4298
DO 10.21873/anticanres.12727
VO 38
IS 7
A1 MASHIBA, RIHO
A1 ISHIKAWA, MAMI
A1 SUMIYA, YU
A1 KAWAKATSU, KUMPEI
A1 TRAN, NGOC KIET
A1 NISHIKATA, TAKAHITO
YR 2018
UL http://ar.iiarjournals.org/content/38/7/4295.abstract
AB Background/Aim: Serum-derived macrophage activating factor (serum MAF) is known to increase the phagocytic activity of macrophages and potentially plays a role in activating cancer immunity. In order to reveal the contributing factors for phagocytic activation, the migratory activity and the efficiency of engulfment was analyzed. Materials and Methods: THP-1 macrophages were induced by 12-O-tetradecanoyl-13-acetate (TPA). The migratory activity and efficiency of engulfment were analyzed by time-lapse imaging and suspension assay, respectively. Results: While the distance of migration did not change before and after activation with serum MAF, the efficiency of beads internalisation was significantly increased. Conclusion: Phagocytic activation of serum-MAF-treated macrophages was caused by increasing the efficiency of engulfment. This study contributes to the knowledge about the activation of the immune system through phagocytic activation of macrophages.