RT Journal Article SR Electronic T1 Deregulation of the SRC Family Tyrosine Kinases in Gastric Carcinogenesis in Non-human Primates JF Anticancer Research JO Anticancer Res FD International Institute of Anticancer Research SP 6317 OP 6320 DO 10.21873/anticanres.12988 VO 38 IS 11 A1 JOANA DE FÁTIMA FERREIRA BORGES DA COSTA A1 CARLA DE CASTRO SANT' ANNA A1 JOSÉ AUGUSTO PEREIRA CARNEIRO MUNIZ A1 CARLOS ALBERTO MACHADO DA ROCHA A1 LETÍCIA MARTINS LAMARÃO A1 CAROLINE DE FÁTIMA AQUINO MOREIRA NUNES A1 PAULO PIMENTEL DE ASSUMPÇÃO A1 ROMMEL RODRIGUEZ BURBANO YR 2018 UL http://ar.iiarjournals.org/content/38/11/6317.abstract AB Background/Aim: The evolution of gastric carcinogenesis remains largely unknown, as the regulatory mechanisms involved in the aggressiveness of gastric cancer are still poorly understood. Kinases are downstream modulators and effectors of various cell signaling cascades and play key roles in the development of neoplastic diseases. The objective of this study was to evaluate the expression of genes and proteins of the SRC family, including FYN, YES, BLK, FGR, LYN and SRC, in a model of intestinal gastric carcinogenesis generated by treating Cebus apella, a New World non-human primate, with N-methyl nitrosourea (MNU). Materials and Methods: mRNA expression of genes was measured by real-time reverse transcription quantitative PCR (RT-qPCR) and protein expression was measured by western blotting in six Cebus apella treated with N-methyl-nitrosourea (MNU) for about 2.5 years. Results: Elevated mRNA and protein expression mainly of the SRC and LYN kinases was observed. Their expression was gradually increasing as non-atrophic gastritis was evolving to gastric cancer. Conclusion: SRC family kinases play a key role in tumor progression and metastasis and may be a promising target for the treatment of gastric cancer.