RT Journal Article
SR Electronic
T1 Colorectal Cancer Cell Line SW480 and SW620 Released Extravascular Vesicles: Focus on Hypoxia-induced Surface Proteome Changes
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 6133
OP 6138
DO 10.21873/anticanres.12965
VO 38
IS 11
A1 ILVA NAKURTE
A1 KASPARS JEKABSONS
A1 REINIS REMBERGS
A1 ELINA ZANDBERGA
A1 ARTURS ABOLS
A1 AIJA LINĒ
A1 RUTA MUCENIECE
YR 2018
UL http://ar.iiarjournals.org/content/38/11/6133.abstract
AB Background/Aim: Extravascular vesicle (EV) proteome closely reflects the proteome of the cell of origin. Therefore, cancer cell-derived EV proteomic analysis could help in identifying cancer biomarkers. This study's goal was to investigate hypoxia-induced proteomic changes in EV released from hypoxic human isogenic non-metastatic colorectal cancer cells SW480 and metastatic colorectal cancer cells SW620. Materials and Methods: EV were characterized by western blot, transmission electron microscopy, proteomic analysis using liquid chromatography time-of-flight-mass spectrometry and quantified by an label-free intensity-based absolute quantitation (iBAQ) approach. Results: A total of 16 proteins in hypoxic EV exceeded normoxic EV protein levels in SW480 EV. Of them, 15 were also found in EV of hypoxic SW620 cells. The expression levels of proteins differed quantitatively: iBAQ (log 10) scores of the levels of five proteins in SW620 EV exceeded those in hypoxic SW480 EV and levels of 11 proteins in SW480 EV exceeded those of SW620 EV. Conclusion: Under hypoxia, colorectal cancer cells release EV that qualitatively and quantitatively change the surface proteome. In the future, the specific hypoxia-induced proteins could be developed as new biomarkers for non-invasive assessment of tumour hypoxia.