RT Journal Article
SR Electronic
T1 Modified Nucleosides – Molecular Markers Suitable for Small-volume Cancer?
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 6113
OP 6119
DO 10.21873/anticanres.12962
VO 38
IS 11
A1 PHILIPP OPITZ
A1 OLF HERBARTH
A1 ANNEROSE SEIDEL
A1 ANDREAS BOEHM
A1 MILOS FISCHER
A1 CHRISTIAN MOZET
A1 ANDREAS DIETZ
A1 GUNNAR WICHMANN
YR 2018
UL http://ar.iiarjournals.org/content/38/11/6113.abstract
AB Background: Modified nucleosides (mNS) in urine are shown to be encouraging markers in cancer, mostly in patients presenting with high tumor mass such is breast and lung cancer. To our knowledge, mNS have not been investigated in head and neck squamous cell carcinoma (HNSCC). HNSCC is characterized by early metastasis into locoregional lymph nodes and slow infiltrating growth, but even in the advanced stage exhibits only a relatively low cancer volume. Therefore, reliable distinction between HNSCC and healthy controls by urinary mNS might pose substantial analytical problems and even more as patients with HNSCC mostly have an increased exposure to tobacco smoke and excessive alcohol consumption which affect the renal mNS pattern. Materials and Methods: Urinary mNS in samples of 93 therapy-naive patients with HNSCC and 242 healthy controls were quantified by reversed-phase high-performance liquid chromatography. Considering that the circadian rhythm causes diuresis-induced variations in concentration, the mNS-to-creatinine ratio was chosen to compare patients and controls. For sensitivity and specificity in discriminating between patients and controls, the corresponding curve was plotted. Additionally, logistic regression was carried out and a multilayer perceptron neuronal network (NN) was created. Results: Fifteen mNS were detectable in cases and controls; concentrations of 11 were found to be significantly different. The sensitivity and specificity depend on the total volume of the lesion; HNSCC with volume <20 ml was reliably detected, but those with a volume of 20 ml or greater produced amounts of mNS which led to the most accurate detection of HNSCC based on HNSCC-specific mNS patterns. Conclusion: Analysis of urinary mNS allows for detection of small-volume HNSCC, with acceptable specificity and sensitivity if the tumor volume exceeds 20 ml.