TY - JOUR T1 - Kisspeptin Inhibits Colorectal Cancer Cell Invasiveness by Activating PKR and PP2A JF - Anticancer Research JO - Anticancer Res SP - 5791 LP - 5798 DO - 10.21873/anticanres.12918 VL - 38 IS - 10 AU - JEONG NAM KIM AU - TAE HUN KIM AU - JI-HYE YOON AU - SUNG-GOOK CHO Y1 - 2018/10/01 UR - http://ar.iiarjournals.org/content/38/10/5791.abstract N2 - Background/Aim: The aim of the present study was to investigate the mechanism through which kisspeptin inhibits colorectal cancer metastasis. Materials and Methods: Colorectal cancer cells were treated with kisspeptin and then subjected to assays for cell viability, migration, invasion and anchorage-independent growth. Kisspeptin receptor (KISS1R) requirement was examined by siRNA-based gene silencing followed by western blot and invasion assays. Kisspeptin regulation of PKR and PP2A was examined by treating cells with inhibitors for PKR or PP2A. Results: Kisspeptin inhibited colorectal cancer cell invasiveness without affecting cell proliferation. Kisspeptin required activation of KISS1R and resulted in activation of PKR and PP2A. PKR inhibitor blocked kisspeptin-induced PP2A phosphorylation, while PP2A inhibitor failed to block kisspeptin-induced PKR phosphorylation. Conclusion: Kisspeptin-mediated activation of PKR-PP2A inhibited colorectal cancer cell invasiveness. ER -