RT Journal Article
SR Electronic
T1 X-ray as Irradiation Alternative for K562 Feeder Cell Inactivation in Human Natural Killer Cell Expansion
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 5767
OP 5772
DO 10.21873/anticanres.12915
VO 38
IS 10
A1 GWANG HO KIM
A1 HOANG-NGUYEN DANG
A1 MINH-TRANG THI PHAN
A1 SOON HO KWEON
A1 SEJONG CHUN
A1 DUCK CHO
YR 2018
UL http://ar.iiarjournals.org/content/38/10/5767.abstract
AB Background/Aim: γ-Irradiation has been proven to be the most effective method to inactivate K562 cells, but γ-irradiators are not available in some institutes. This study was designed to compare the effects of X-ray and γ-irradiation on K562 cells in natural killer (NK) cell expansion. Materials and Methods: To expand NK cells, isolated peripheral blood mononuclear cells (PBMCs) were co-cultured with γ-irradiated or X-ray-treated K562 cells plus IL-2 and IL-15. Characteristics of expanded NK cells were identified by flow cytometry. Results: NK cell expansion rate tended be to lower in the X-ray-treated group (68.9±32.6) than the γ-irradiated group (78±28.7), but the difference was not significant (p=0.39). Furthermore, NK cell functions or receptor expression were similar in the two groups. Conclusion: Our results suggest that X-ray treatment can be used as an alternative to γ-irradiation for K562 cells inactivation in human NK cell expansion.