@article {KIM5767, author = {GWANG HO KIM and HOANG-NGUYEN DANG and MINH-TRANG THI PHAN and SOON HO KWEON and SEJONG CHUN and DUCK CHO}, title = {X-ray as Irradiation Alternative for K562 Feeder Cell Inactivation in Human Natural Killer Cell Expansion}, volume = {38}, number = {10}, pages = {5767--5772}, year = {2018}, doi = {10.21873/anticanres.12915}, publisher = {International Institute of Anticancer Research}, abstract = {Background/Aim: γ-Irradiation has been proven to be the most effective method to inactivate K562 cells, but γ-irradiators are not available in some institutes. This study was designed to compare the effects of X-ray and γ-irradiation on K562 cells in natural killer (NK) cell expansion. Materials and Methods: To expand NK cells, isolated peripheral blood mononuclear cells (PBMCs) were co-cultured with γ-irradiated or X-ray-treated K562 cells plus IL-2 and IL-15. Characteristics of expanded NK cells were identified by flow cytometry. Results: NK cell expansion rate tended be to lower in the X-ray-treated group (68.9{\textpm}32.6) than the γ-irradiated group (78{\textpm}28.7), but the difference was not significant (p=0.39). Furthermore, NK cell functions or receptor expression were similar in the two groups. Conclusion: Our results suggest that X-ray treatment can be used as an alternative to γ-irradiation for K562 cells inactivation in human NK cell expansion.}, issn = {0250-7005}, URL = {https://ar.iiarjournals.org/content/38/10/5767}, eprint = {https://ar.iiarjournals.org/content/38/10/5767.full.pdf}, journal = {Anticancer Research} }