RT Journal Article
SR Electronic
T1 Cold Physical Plasma Selectively Elicits Apoptosis in Murine Pancreatic Cancer Cells <em>In Vitro</em> and <em>In Ovo</em>
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 5655
OP 5663
DO 10.21873/anticanres.12901
VO 38
IS 10
A1 KIM ROUVEN LIEDTKE
A1 STEPHAN DIEDRICH
A1 ONUR PATI
A1 ERIC FREUND
A1 ROBERT FLIEGER
A1 CLAUS DIETER HEIDECKE
A1 LARS IVO PARTECKE
A1 SANDER BEKESCHUS
YR 2018
UL http://ar.iiarjournals.org/content/38/10/5655.abstract
AB Background/Aim: Poor prognosis of pancreatic cancer has remained almost unchanged in recent years. Cold physical plasma was suggested as an innovative anticancer strategy, but its selective killing activity of malignant over non-malignant cells has only partially been explored. The present study aimed at exploring the effect of cold physical plasma on cellular viability. Materials and Methods: Induction of cell death and apoptosis by cold physical plasma was investigated in murine PDA6606 pancreatic cancer cells and primary murine fibroblasts in vitro (2D and 3D cultures) and in ovo. Results: Plasma increased apoptosis in PDA6606 to a significantly higher extent compared to fibroblasts. Antioxidants abrogated these effects, suggesting a prime role of reactive oxygen species in plasma-induced apoptosis. Plasma increased apoptosis of 3D PDA6606 multicellular spheres grown in vitro and in ovo, to significantly higher rates compared to that of fibroblasts, with minimum in ovo inflammation or necrosis observed by hematoxylin and eosin staining (H&amp;E). Conclusion: These data support the future intra-operative application of cold physical plasma for the treatment of microscopic residual tumor tissue after surgical resection.