@article {KONDO5923, author = {YASUSHI KONDO and TAKASHI FUKUYAMA and RUI YAMAMURA and NOBUE FUTAWATARI and YOSHINOBU ICHIKI and YOICHI TANAKA and YATSUSHI NISHI and YOSHIHITO TAKAHASHI and HITOSHI YAMAZAKI and NORITADA KOBAYASHI and MASAHIKO WATANABE}, title = {Detection of KK-LC-1 Protein, a Cancer/Testis Antigen, in Patients with Breast Cancer}, volume = {38}, number = {10}, pages = {5923--5928}, year = {2018}, doi = {10.21873/anticanres.12937}, publisher = {International Institute of Anticancer Research}, abstract = {Background: Kita-Kyushu lung cancer antigen-1 (KK-LC-1) is a cancer/testis antigen and predominant target for cancer immunotherapy. Its detection is only established based on gene expression. In this study, we established a monoclonal antibody against KK-LC-1 to detect its protein expression in formalin-fixed samples. Materials and Methods: The monoclonal antibody against KK-LC-1 was evaluated and the detection of KK-LC-1 between gene expression and protein expression was compared in patients with breast cancer. The monoclonal antibody clone 34B3, which we established, stained testicular germ cells positively. Results: The rates of detection of KK-LC-1 gene and protein expression were 11.8\% and 52.9\%, respectively. Protein expression was detected in all triple-negative breast cancer cases studied (n=8). Furthermore, KK-LC-1 was detected in all tumours without oestrogen receptor expression. Conclusion: This study indicated that KK-LC-1 expression was detected in breast cancer, especially in oestrogen receptor-negative subtypes.}, issn = {0250-7005}, URL = {https://ar.iiarjournals.org/content/38/10/5923}, eprint = {https://ar.iiarjournals.org/content/38/10/5923.full.pdf}, journal = {Anticancer Research} }