RT Journal Article
SR Electronic
T1 Up-regulation of Bone Morphogenetic Protein 7 by 2-Hydroxycinnamaldehyde Attenuates HNSCC Cell Invasion
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 5747
OP 5757
DO 10.21873/anticanres.12913
VO 38
IS 10
A1 SUNG-MIN KANG
A1 JINKYUNG KIM
A1 SOO HYUN KANG
A1 SU YOUNG OH
A1 HEON-JIN LEE
A1 BYOUNG-MOG KWON
A1 SU-HYUNG HONG
YR 2018
UL http://ar.iiarjournals.org/content/38/10/5747.abstract
AB Background/Aim: Few studies have examined the effect of 2’-hydroxycinnamaldehyde (HCA) on head and neck squamous cell carcinoma (HNSCC) cell invasion. This study examined the role of BMP7 on the anti-migration and anti-invasion activity of HCA using HNSCC cells. Materials and Methods: Matrigel invasion and wound healing assays were conducted to investigate cell migration or invasion. BMP7 overexpression vector or siRNA mixture was used for transient regulation of gene expression. Results: HCA attenuated HNSCC cell migration and spheroids Matrigel invasion without cytotoxicity. mRNA and protein expression of BMP7 increased with HCA treatment. Exogenous BMP7 overexpression without HCA treatment attenuated Matrigel invasion of cells. Furthermore, suppression of BMP7 by siRNA alleviated the inhibitory effect of HCA on the invasion of Matrigel by the cell, indicating that BMP7 is responsible for the anti-migration effect of HCA in HNSCC cells. Conclusion: HCA treatment led to a remarkable up-regulation of BMP7, which resulted in the attenuation of HNSCC cell invasion.