%0 Journal Article %A TAKAKI AKAMINE %A YOSUKE MORODOMI %A YUI HARADA %A KOJI TERAISHI %A TETSUZO TAGAWA %A TATSURO OKAMOTO %A YOSHIHIKO MAEHARA %A YOSHIKAZU YONEMITSU %T miR-3148 Is a Novel Onco-microRNA that Potentiates Tumor Growth In Vivo %D 2018 %R 10.21873/anticanres.12906 %J Anticancer Research %P 5693-5701 %V 38 %N 10 %X Background/Aim: Alterations of microRNA expression in three-dimensional spheroids were examined to identify novel microRNAs that might be associated with tumorigenesis. Materials and Methods: Using microRNA microarray analysis, we screened for microRNAs that were dramatically up-regulated inside three-dimensional spheroids in genetically-modified HCT116 human colon cancer cells expressing Copepoda Green Fluorescent Protein under hypoxia. Results: miR-3148 was identified as a possible candidate onco-microRNA. A growth advantage of HCT116 cells stably expressing miR-3148 (HCT116-miR3148) was observed compared to parental cells in vivo, but not in vitro. Additionally, no change in growth under hypoxic or starvation conditions was seen in these cells cultured two-dimensionally; however, HCT116-miR3148 cells maintained as three-dimensional spheroids were highly resistant to hypoxic conditions. HCT116-miR3148 cells were more sensitive to mitogen-activated protein kinase (MAPK) kinase inhibitors and extracellular signal-regulated kinase (ERK) inhibitors. Conclusion: MiR-3148 may be a novel onco-microRNA that protects cancer cells from serious stress conditions through the MAPK/ERK pathway, especially in vivo. %U https://ar.iiarjournals.org/content/anticanres/38/10/5693.full.pdf