TY - JOUR T1 - <em>Trichomonas Vaginalis</em> Inhibits HeLa Cell Growth Through Modulation of Critical Molecules for Cell Proliferation and Apoptosis JF - Anticancer Research JO - Anticancer Res SP - 5079 LP - 5086 DO - 10.21873/anticanres.12827 VL - 38 IS - 9 AU - ZIWEN ZHU AU - LEI ZHAO AU - ANDREW BRITTINGHAM AU - QIAN BAI AU - MARK R. WAKEFIELD AU - YUJIANG FANG Y1 - 2018/09/01 UR - http://ar.iiarjournals.org/content/38/9/5079.abstract N2 - Background/Aim: Cervical cancer is one of the deadliest gynecological cancers in USA. The role of Trichomonas Vaginalis (T. Vag) in the etiology or pathogenesis of cervical cancer is still poorly understood and controversial. Materials and Methods: Clonogenic assay, PCNA staining, TUNEL staining and caspase-3 activity assay were used to investigate the direct in vitro effect of T. Vag on human cervical cancer by using HeLa cells. We further investigated the potential molecular mechanisms using RT-PCR and immunohistochemical staining. Results: We found that culture supernatant of T. Vag inhibited growth of HeLa cervical cancer cells and this correlated with up-regulation of p15. We also found that culture supernatant of T. Vag induced apoptosis of HeLa cells and this correlated with up-regulation of Fas, TRAIL and TRAILR1. Conclusion: Culture supernatant of T. Vag inhibits growth of HeLa cervical cancer cells by inhibition of proliferation and promotion of apoptosis. Our study might be helpful to address the association between the development of cervical cancer and infection of T. Vag. ER -