PT - JOURNAL ARTICLE AU - A. H. M. ZUBERI ASHRAF AU - SYEDA H. AFROZE AU - KOSEI YAMAUCHI AU - DAVID C. ZAWIEJA AU - THOMAS J. KEUHL AU - LAURA WEISER ERLANDSON AU - MOHAMMAD N. UDDIN TI - Differential Mechanism of Action of 3,4’,7-O-trimethylquercetin in Three Types of Ovarian Cancer Cells AID - 10.21873/anticanres.12835 DP - 2018 Sep 01 TA - Anticancer Research PG - 5131--5137 VI - 38 IP - 9 4099 - http://ar.iiarjournals.org/content/38/9/5131.short 4100 - http://ar.iiarjournals.org/content/38/9/5131.full SO - Anticancer Res2018 Sep 01; 38 AB - Background/Aim: 3,4’,7-O-trimethylquercetin (34’7TMQ), a derivative of quercetin, inhibited ovarian cancer cell migration and invasion without affecting proliferation. In this study, the apoptotic effect of 34’7TMQ on three cancer cell lines (CRL-1978, CRL-11731, SK-OV-3) was evaluated. Materials and Methods: Expression of pro-apoptotic proteins such as Bax/Bcl-2 ratio, p38 MAP kinase, and caspase-9 were measured by western blot analysis. Annexin-V staining was performed to visualize apoptotic signaling. Results: Caspase-9 was up-regulated in all three cell lines. Bax/Bcl-2 ratio was up-regulated in CRL-1978 and SK-OV-3 but down-regulated in CRL-11731. The p38 MAPK was down-regulated in CRL-1978, up-regulated in SK-OV-3, and had differential expression in CRL-11731. Annexin V staining indicated that 34’7TMQ at 6.25 μM induced apoptotic signaling in the CRL-1978 ovarian cancer cell line. Conclusion: 34’7TMQ induced apoptosis in three types of cancer cell lines but it appears to have a different mechanism of action in each cell line.