@article {ASHRAF5131, author = {A. H. M. ZUBERI ASHRAF and SYEDA H. AFROZE and KOSEI YAMAUCHI and DAVID C. ZAWIEJA and THOMAS J. KEUHL and LAURA WEISER ERLANDSON and MOHAMMAD N. UDDIN}, title = {Differential Mechanism of Action of 3,4{\textquoteright},7-O-trimethylquercetin in Three Types of Ovarian Cancer Cells}, volume = {38}, number = {9}, pages = {5131--5137}, year = {2018}, doi = {10.21873/anticanres.12835}, publisher = {International Institute of Anticancer Research}, abstract = {Background/Aim: 3,4{\textquoteright},7-O-trimethylquercetin (34{\textquoteright}7TMQ), a derivative of quercetin, inhibited ovarian cancer cell migration and invasion without affecting proliferation. In this study, the apoptotic effect of 34{\textquoteright}7TMQ on three cancer cell lines (CRL-1978, CRL-11731, SK-OV-3) was evaluated. Materials and Methods: Expression of pro-apoptotic proteins such as Bax/Bcl-2 ratio, p38 MAP kinase, and caspase-9 were measured by western blot analysis. Annexin-V staining was performed to visualize apoptotic signaling. Results: Caspase-9 was up-regulated in all three cell lines. Bax/Bcl-2 ratio was up-regulated in CRL-1978 and SK-OV-3 but down-regulated in CRL-11731. The p38 MAPK was down-regulated in CRL-1978, up-regulated in SK-OV-3, and had differential expression in CRL-11731. Annexin V staining indicated that 34{\textquoteright}7TMQ at 6.25 μM induced apoptotic signaling in the CRL-1978 ovarian cancer cell line. Conclusion: 34{\textquoteright}7TMQ induced apoptosis in three types of cancer cell lines but it appears to have a different mechanism of action in each cell line.}, issn = {0250-7005}, URL = {https://ar.iiarjournals.org/content/38/9/5131}, eprint = {https://ar.iiarjournals.org/content/38/9/5131.full.pdf}, journal = {Anticancer Research} }