PT - JOURNAL ARTICLE AU - NATSUKO FUKUOKA AU - OSAMU NAKAMURA AU - YOSHIKI YAMAGAMI AU - HIDEKI NISHIMURA AU - YOICHI ISHIBASHI AU - TETSUJI YAMAMOTO TI - SNX-2112 Induces Apoptosis and Autophagy of Nara-H Cells AID - 10.21873/anticanres.12840 DP - 2018 Sep 01 TA - Anticancer Research PG - 5177--5181 VI - 38 IP - 9 4099 - http://ar.iiarjournals.org/content/38/9/5177.short 4100 - http://ar.iiarjournals.org/content/38/9/5177.full SO - Anticancer Res2018 Sep 01; 38 AB - Background/Aim: Selective heat shock protein 90 (Hsp90) inhibitor SNX-2112 exhibits antitumor activity in multiple cancer cell types. Here, the antitumor activity of SNX-2112 in Nara-H cells was analyzed. Materials and Methods: Antitumor activity of SNX-2112 was assessed using a cell proliferation assay. We also examined the signalling pathways involved in SNX-2112-mediated autophagy and apoptosis of Nara-H cells by western blot and morphological analyses. Results: Cell proliferation assays demonstrated that SNX-2112 inhibited Nara-H cell growth. Western blotting revealed that SNX-2112 induced apoptosis and autophagy, inhibited mammalian target of rapamycin (mTOR) phosphorylation, and suppressed the mitogen-activated protein kinase (MAPK) signalling pathway. Morphological analysis confirmed that SNX-2112 induced autophagy and apoptosis. Conclusion: SNX-2112 induced autophagy and apoptosis of Nara-H cells by inhibiting mTOR and MAPK pathways. Our results support developing SNX-2112 to treat human soft tissue sarcomas.