%0 Journal Article %A JEEHAN LEE %A HYE HYEON YUN %A SEULKI KIM %A SANG HEE JI %A HYO-JEONG KUH %A JEONG-HWA LEE %T Implication of BIS in the Migration and Invasion of A549 Non-small Cell Lung Cancer Cells %D 2018 %R 10.21873/anticanres.12756 %J Anticancer Research %P 4525-4533 %V 38 %N 8 %X Background/Aim: High expression of the Bcl-2-interacting cell death suppressor (BIS), an anti-apoptotic protein, in various human cancers is linked to a poor outcome. The purpose of this study was to clarify whether BIS is associated with the migration and invasive characteristics of A549 cells. Materials and Methods: BIS-knockout (KO) cells were prepared by the CRISPR/Cas9 method. The aggressive behaviors of A549 cells were analyzed by wound healing and a transwell invasion assay as well as 3D spheroid culture. Results: BIS depletion resulted in significant inhibition of the migration and invasive potential of A549 cells which was accompanied by an increased ratio of E-cadherin/N-cadherin and a decrease in the mRNA levels of Zeb1, Snail, Slug and MMP-2. NF-κB activity was suppressed in BIS-KO A549 cells due to the decrease in p65 protein levels, but not in mRNA levels. Conclusion: BIS regulates cell invasion and the induction of the epithelial-mesenchymal transition (EMT) phenotype in A549 cells probably via the NF-κB signaling pathway. %U https://ar.iiarjournals.org/content/anticanres/38/8/4525.full.pdf