RT Journal Article
SR Electronic
T1 DL-Methadone as an Enhancer of Chemotherapeutic Drugs in Head and Neck Cancer Cell Lines
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 3633
OP 3639
DO 10.21873/anticanres.13511
VO 39
IS 7
A1 LANDGRAF, VERONIKA
A1 GRIESSMANN, MATTHIAS
A1 ROLLER, JEANETTE
A1 POLEDNIK, CHRISTINE
A1 SCHMIDT, MARIANNE
YR 2019
UL http://ar.iiarjournals.org/content/39/7/3633.abstract
AB Background/Aim: The aim of this study was to analyze the effect of DL-methadone on enhancing the action of the chemotherapeutic drugs cisplatin, doxorubicin, 5-fluoruracil (5-FU) and paclitaxel on head and neck squamous carcinoma (HNSCC) cell lines. Materials and Methods: The chemotherapeutic drugs were applied alone or in combination with DL-methadone and cytotoxicity was analyzed by XTT assays. Expression of the μ-opioid receptor and the drug transporter p-glycoprotein were analyzed by qRT-PCR. Results: The effect of DL-methadone strongly depended on the respective chemotherapeutic agent. The basic expression of the μ-opioid receptor was not associated with the effect of DL-methadone, rather its induction by chemotherapeutic drugs. Expression or expression induction of p-glycoprotein was higher in weak-responder cell lines. Conclusion: Enhancement of the toxicity of chemotherapeutic drugs by DL-methadone depends on the drug and on the cell line used.