TY - JOUR T1 - 2-Deoxy-glucose Enhances the Effect of Cisplatin and Pemetrexed in Reducing Malignant Pleural Mesothelioma Cell Proliferation But Not Spheroid Growth JF - Anticancer Research JO - Anticancer Res SP - 3809 LP - 3814 DO - 10.21873/anticanres.13530 VL - 39 IS - 7 AU - IRINI GEROGIANNI AU - ELEANNA PITARAKI AU - RAJESH M. JAGIRDAR AU - OLYMPIA KOULIOU AU - LYDIA GIANNAKOU AU - STEFANOS GIANNOPOULOS AU - ELEFTHERIOS PAPAZOGLOU AU - CHRISSI HATZOGLOU AU - KONSTANTINOS I. GOURGOULIANIS AU - SOTIRIOS G. ZAROGIANNIS Y1 - 2019/07/01 UR - http://ar.iiarjournals.org/content/39/7/3809.abstract N2 - Background/Aim: Malignant pleural mesothelioma (MPM) is a therapy-resistant neoplasm of the pleura. Standard chemotherapy consists of a combination of cisplatin (CPDD) and pemetrexed (PEM). The aim of this study was to assess whether inhibition of aerobic glycolysis by 2-deoxy-glucose (2DG) would enhance the effects of standard chemotherapy. Materials and Methods: MeT-5A, M14K, MSTO and ZL34 cell lines were used. Cell viability with 2DG and cell proliferation and spheroid formation with CPDD+PEM alone and with 2-DG were tested. Results: Viability with 2-DG was dose-dependent. Cell proliferation with CPDD+PEM on 2D surface was reduced in all cell types, 2-DG inclusion demonstrated a synergistic effect in MSTO and ZL34 cells. Spheroid growth in 3D with CPDD+PEM or CPDD+PEM+2-DG lowered spheroid growth in all cell types. Conclusion: 2-DG synergizes with CPDD+PEM in lowering MPM cell proliferation in 2D to <20%. In 3D MPM spheroid growth 2-DG synergism with CPDD+PEM treatment is not maintained. ER -