RT Journal Article
SR Electronic
T1 RAW 264.7 Macrophage Polarization by Pancreatic Cancer Cells – A Model for Studying Tumour-promoting Macrophages
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 2871
OP 2882
DO 10.21873/anticanres.13416
VO 39
IS 6
A1 AYDAR KHABIPOV
A1 ANDRE KÄDING
A1 KIM ROUVEN LIEDTKE
A1 ERIC FREUND
A1 LARS-IVO PARTECKE
A1 SANDER BEKESCHUS
YR 2019
UL http://ar.iiarjournals.org/content/39/6/2871.abstract
AB Background/Aim: Tumour-associated macrophages (TAMs) are highjacked M2-polarized macrophages that especially promote pancreatic cancer growth. The aim of this study was to identify an easy-to-use cell culture model suitable for studying this interaction and macrophage polarization. Materials and Methods: Co-cultures of two cell lines, PDA6606 cells with RAW macrophages cells were used in vitro and in ovo. Macrophages were analyzed by microscopy, magnetic resonance imaging (MRI), and flow cytometry. Results: By comparing chemically-induced M1 and M2 macrophages, a clear induction of the M2 phenotype of RAW macrophages by PDA6606 pancreatic cancer cells was observed in vitro. In ovo, PDA6606 cells and conditioned media polarized macrophages to the M2 phenotype, which in turn promoted tumour growth and angiogenesis via their surface marker profiles and VEGF production. Conclusion: PDA6606 pancreatic cancer cells expectantly and potently induced M2 polarization of RAW264.7 macrophages. This model may be used to study pancreatic cancer-macrophage plasticity in e.g. drug research in vitro and in ovo.