TY - JOUR T1 - MEKK3 Sustains EMT and Stemness in Pancreatic Cancer by Regulating YAP and TAZ Transcriptional Activity JF - Anticancer Research JO - Anticancer Res SP - 1937 LP - 1946 VL - 38 IS - 4 AU - RAFFAELA SANTORO AU - MARCO ZANOTTO AU - CARMINE CARBONE AU - GENY PIRO AU - GIAMPAOLO TORTORA AU - DAVIDE MELISI Y1 - 2018/04/01 UR - http://ar.iiarjournals.org/content/38/4/1937.abstract N2 - Background/Aim: Pancreatic cancer is one of the most threatening and poorly understood human malignancies. MEKK3 (MAP3K3) is a serine/threonine kinase activated by different signaling pathways. YAP and TAZ are critical oncogenic effectors in pancreatic cancer. We hypothesized that MEKK3 could sustain pancreatic cancer by inducing YAP/TAZ oncogenic activities. Materials and Methods: In Panc1 and AsPC1 pancreatic cancer cell lines MEKK3 was knocked-out (KO) by the CRISPR/Cas9 method. These cells were used to evaluate MEKK3 contribution to the expression of YAP/TAZ and their target genes, cell migration, stemness, and in vivo tumor growth. Results: MEKK3 KO reduced both EMT and cell migration, the size of 3D colonies and the percentage of CD44+/CD24+/EpCAM+ CSC, promoter recruitment of YAP/TAZ and the expression of their target genes. It reduced tumor growth and prolonged mice overall survival. Conclusion: Silencing of MEKK3 represents a valid approach to revert in vivo the aggressiveness of pancreatic cancer by modulating YAP/TAZ transcriptional activities. ER -