TY - JOUR T1 - Altered Polyamine Profiles in Colorectal Cancer JF - Anticancer Research JO - Anticancer Res SP - 3601 LP - 3607 DO - 10.21873/anticanres.12634 VL - 38 IS - 6 AU - MARKUS K. VENÄLÄINEN AU - ANTTI N. ROINE AU - MERJA R. HÄKKINEN AU - JOUKO J. VEPSÄLÄINEN AU - PEKKA S. KUMPULAINEN AU - MIKKO S. KIVINIEMI AU - TERHO LEHTIMÄKI AU - NIKU K. OKSALA AU - TUOMO K. RANTANEN Y1 - 2018/06/01 UR - http://ar.iiarjournals.org/content/38/6/3601.abstract N2 - Background: The declining mortality rate of patients with colorectal cancer (CRC) can be explained, at least partially, with early diagnosis. Simple diagnostic methods are needed to achieve a maximal patient participation rate in screening. Materials and Methods: Liquid chromatography electrospray tandem mass spectrometry (LC-MS/MS) was used to determine urinary polyamine (PA) profiles. In a prospective setting, 116 patients were included in the study: 57 with CRC, 13 with inflammatory bowel disease (IBD), 12 with adenoma, and 34 controls. Results: N1,N12-diacetylspermine (DiAcSPM) level was significantly higher in patients with CRC than controls (sensitivity=78.0%, specificity=70.6%; p=0.00049). The level of diacetylated cadaverine (p=0.0068) was lower and that of diacetylated putrescine (p=0.0078) was higher in patients with CRC than in those with IBD. Cadaverine (p=0.00010) and spermine (p=0.042) levels were lower and that of DiAcSPM (p=0.018) higher in patients with CRC than in those with adenoma. Conclusion: The simultaneous determination of urinary PAs by means of LC-MS/MS can be used to discriminate CRC from controls and patients with benign colorectal diseases. ER -