RT Journal Article SR Electronic T1 Detection of Distinct Changes in Gene-expression Profiles in Specimens of Tumors and Transition Zones of Tenascin-positive/-negative Head and Neck Squamous Cell Carcinoma JF Anticancer Research JO Anticancer Res FD International Institute of Anticancer Research SP 1279 OP 1290 VO 38 IS 3 A1 VERONIKA ZIVICOVA A1 PETER GAL A1 ALZBETA MIFKOVA A1 STEPAN NOVAK A1 HERBERT KALTNER A1 MICHAL KOLAR A1 HYNEK STRNAD A1 JANA SACHOVA A1 MILUSE HRADILOVA A1 MARTIN CHOVANEC A1 HANS-JOACHIM GABIUS A1 KAREL SMETANA, JR. A1 ZDENEK FIK YR 2018 UL http://ar.iiarjournals.org/content/38/3/1279.abstract AB Background/Aim: Having previously initiated genome-wide expression profiling in head and neck squamous cell carcinoma (HNSCC) for regions of the tumor, the margin of surgical resecate (MSR) and normal mucosa (NM), we here proceed with respective analysis of cases after stratification according to the expression status of tenascin (Ten). Materials and Methods: Tissue specimens of each anatomical site were analyzed by immunofluorescent detection of Ten, fibronectin (Fn) and galectin-1 (Gal-1) as well as by microarrays. Results: Histopathological examination demonstrated that Ten+Fn+Gal-1+ co-expression occurs more frequently in samples of HNSCC (55%) than in NM (9%; p<0.01). Contrary, the Ten−Fn+Gal-1− (45%) and Ten−Fn−Gal-1− (39%) status occurred with significantly (p<0.01) higher frequency than in HNSCC (3% and 4%, respectively). In MSRs, different immunophenotypes were distributed rather equally (Ten+Fn+Gal-1+=24%; Ten−Fn+Gal-1−=36%; Ten−Fn−Gal-1−=33%), differing to the results in tumors (p<0.05). Absence/presence of Ten was used for stratification of patients into cohorts without a difference in prognosis, to comparatively examine gene-activity signatures. Microarray analysis revealed i) expression of several tumor progression-associated genes in Ten+ HNSCC tumors and ii) a strong up-regulation of gene expression assigned to lipid metabolism in MSRs of Ten− tumors, while NM profiles remained similar. Conclusion: The presented data reveal marked and specific changes in tumors and MSR specimens of HNSCC without a separation based on prognosis.