RT Journal Article
SR Electronic
T1 Curcumin and Rutin Down-regulate Cyclooxygenase-2 and Reduce Tumor-associated Inflammation in HPV16-Transgenic Mice
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 1461
OP 1466
VO 38
IS 3
A1 MOUTINHO, MAGDA S.S.
A1 ARAGÃO, SOFIA
A1 CARMO, DIOGO
A1 CASACA, FÁTIMA
A1 SILVA, SANDRA
A1 RIBEIRO, JOANA
A1 SOUSA, HUGO
A1 PIRES, ISABEL
A1 QUEIROGA, FELISBINA
A1 COLAÇO, BRUNO
A1 MEDEIROS, RUI
A1 OLIVEIRA, PAULA A.
A1 LOPES, CARLOS
A1 BASTOS, MARGARIDA M.S.M.
A1 DA COSTA, RUI M. GIL
YR 2018
UL http://ar.iiarjournals.org/content/38/3/1461.abstract
AB Aim: Cyclo-oxygenase-2 (COX2) plays a prominent role in carcinogenesis. This study addresses the effects of two nutraceutical compounds on the expression of COX2 and tumor-associated inflammation in human papillomavirus type 16 (HPV16)-transgenic mice. Materials and Methods: Six-week-old FVB/n mice were supplemented with rutin or curcumin for 24 weeks: HPV16−/− no treatment, n=12; HPV16+/− no treatment, n=13; HPV16+/− rutin, n=12; HPV16+/− curcumin, n=13. HPV16-induced skin lesions and their inflammatory infiltrates were studied histologically. COX2 expression was assessed immunohistochemically. Results: Rutin reduced COX2 expression in the dermis (immunostaining score 7.83 versus 11.25 in untreated HPV16-transgenic mice) and epidermis (4.5 versus 10.0). Curcumin led to dermal and epidermal scores of 10.5 and 4.5. Both compounds reduced leukocytic infiltration, but neither prevented epidermal dysplasia. Conclusion: COX2 expression in HPV16-induced lesions may be modulated by nutraceuticals, reducing tumor-associated inflammation. However, this was not sufficient to block carcinogenesis, calling for additional studies focused on combination therapies.