TY - JOUR T1 - Curcumin and Rutin Down-regulate Cyclooxygenase-2 and Reduce Tumor-associated Inflammation in HPV16-Transgenic Mice JF - Anticancer Research JO - Anticancer Res SP - 1461 LP - 1466 VL - 38 IS - 3 AU - MAGDA S.S. MOUTINHO AU - SOFIA ARAGÃO AU - DIOGO CARMO AU - FÁTIMA CASACA AU - SANDRA SILVA AU - JOANA RIBEIRO AU - HUGO SOUSA AU - ISABEL PIRES AU - FELISBINA QUEIROGA AU - BRUNO COLAÇO AU - RUI MEDEIROS AU - PAULA A. OLIVEIRA AU - CARLOS LOPES AU - MARGARIDA M.S.M. BASTOS AU - RUI M. GIL DA COSTA Y1 - 2018/03/01 UR - http://ar.iiarjournals.org/content/38/3/1461.abstract N2 - Aim: Cyclo-oxygenase-2 (COX2) plays a prominent role in carcinogenesis. This study addresses the effects of two nutraceutical compounds on the expression of COX2 and tumor-associated inflammation in human papillomavirus type 16 (HPV16)-transgenic mice. Materials and Methods: Six-week-old FVB/n mice were supplemented with rutin or curcumin for 24 weeks: HPV16−/− no treatment, n=12; HPV16+/− no treatment, n=13; HPV16+/− rutin, n=12; HPV16+/− curcumin, n=13. HPV16-induced skin lesions and their inflammatory infiltrates were studied histologically. COX2 expression was assessed immunohistochemically. Results: Rutin reduced COX2 expression in the dermis (immunostaining score 7.83 versus 11.25 in untreated HPV16-transgenic mice) and epidermis (4.5 versus 10.0). Curcumin led to dermal and epidermal scores of 10.5 and 4.5. Both compounds reduced leukocytic infiltration, but neither prevented epidermal dysplasia. Conclusion: COX2 expression in HPV16-induced lesions may be modulated by nutraceuticals, reducing tumor-associated inflammation. However, this was not sufficient to block carcinogenesis, calling for additional studies focused on combination therapies. ER -