RT Journal Article
SR Electronic
T1 Antitumor Effect of Burchellin Derivatives Against Neuroblastoma
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 855
OP 862
VO 38
IS 2
A1 MASAHIRO KURITA
A1 TOMOMI TAKADA
A1 NORIKO WAKABAYASHI
A1 SATORU ASAMI
A1 SHINICHI ONO
A1 TAKETO UCHIYAMA
A1 TAKASHI SUZUKI
YR 2018
UL http://ar.iiarjournals.org/content/38/2/855.abstract
AB Background: Neuroblastoma is one of the most commonly encountered malignant solid tumors in the pediatric age group. We examined the antitumor effects of five burchellin derivatives against human neuroblastoma cell lines. Materials and Methods: We evaluated cytotoxicity by the MTT assay for four human neuroblastoma and two normal cell lines. We also performed analysis of the apoptotic induction effect by flow cytometry, and examined the expression levels of apoptosis- and cell growth-related proteins by western blot analysis. Results: We found that one of the burchellin derivatives (compound 4) exerted cytotoxicity against the neuroblastoma cell lines. Compound 4 induced caspase-dependent apoptosis via a mitochondrial pathway. The apoptosis mechanisms induced by compound 4 involved caspase-3, -7 and -9 activation and poly (ADP-ribose) polymerase cleavage. In addition, compound 4 induced cell death through inhibition of the cell growth pathway (via extracellular signal-regulated kinase 1 and 2, AKT8 virus oncogene cellular homolog, and signal transducer and activator of transcription 3). Conclusion: Compound 4 exerted cellular cytotoxicity against neuroblastoma cells via induction of caspase-dependent apoptosis, and may offer promise for further development as a useful drug for the treatment of advanced neuroblastoma.