PT  - JOURNAL ARTICLE
AU  - ANIKA C. BUNDSCHERER
AU  - MANUELA MALSY
AU  - MICHAEL A. GRUBER
AU  - BERNHARD M. GRAF
AU  - BARBARA SINNER
TI  - Acetaminophen and Metamizole Induce Apoptosis in HT 29 and SW 480 Colon Carcinoma Cell Lines <em>In Vitro</em>
DP  - 2018 Feb 01
TA  - Anticancer Research
PG  - 745--751
VI  - 38
IP  - 2
4099  - http://ar.iiarjournals.org/content/38/2/745.short
4100  - http://ar.iiarjournals.org/content/38/2/745.full
SO  - Anticancer Res2018 Feb 01; 38
AB  - Background/Aim: The perioperative phase is supposed to be a period with high vulnerability for cancer dissemination. Acetaminophen and metamizole are common analgesics administered during this phase. We investigated the effect of acetaminophen, metamizole and 4-methylaminoantipyrine (MAA) on proliferation and apoptosis of colon carcinoma cell lines (SW 480 and HT 29). Materials and Methods: Proliferation was detected by cell proliferation ELISA BrdU, and apoptosis by Annexin V staining. Cytochrome c and caspase 3, 8 and 9 expression levels were detected by western blot. Results: Acetaminophen, metamizole or MAA caused slight changes in proliferation. Acetaminophen, metamizole or the combination increased apoptosis in both cell lines. All agents decreased caspase 3 and 8 expression in SW480. Acetaminophen decreased caspase 9 expression in both cell lines. Conclusion: In clinically relevant doses, acetaminophen and/or metamizole induce apoptosis in both colon cancer cell lines. Both mitochondrial and death receptor pathways might be involved in acetaminophen-induced apoptosis.