RT Journal Article
SR Electronic
T1 MicroRNA Expression in KRAS- and BRAF-mutated Colorectal Cancers
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 677
OP 683
VO 38
IS 2
A1 IDA V. LUNDBERG
A1 MARIA L. WIKBERG
A1 INGRID LJUSLINDER
A1 XINGRU LI
A1 ROBIN MYTE
A1 CARL ZINGMARK
A1 ANNA LÖFGREN-BURSTRÖM
A1 SOFIA EDIN
A1 RICHARD PALMQVIST
YR 2018
UL http://ar.iiarjournals.org/content/38/2/677.abstract
AB Background/Aim: KRAS and BRAF are two genes commonly mutated in colorectal cancer (CRC). Even though BRAF is a downstream target of KRAS in the MAPK signalling pathway, KRAS- and BRAF-mutated CRCs are found to display several different clinical and histopathological features. We investigated whether a differential expression of microRNAs (miRNAs) could explain the clinicopathological differences seen between KRAS- and BRAF-mutated CRCs. Materials and Methods: Using a PCR array, we analyzed the expression of 84 different miRNAs in CRC cell lines wild-type in KRAS and BRAF, or mutated in KRAS or BRAF. Results: Ten miRNAs were selected for further analyses in tumor tissue specimens (let-7a, let-7i, miR-10a, miR-10b, miR-31, miR-100, miR-181a, miR-181b, miR-372, and miR-373). BRAF-mutated tumors were found to express significantly higher levels of miR-31 as well as significantly lower levels of miR-373, compared to wild-type tumors. Conclusion: Our results suggest that KRAS- and BRAF-mutated CRCs may have different miRNA signatures compared to CRC tumors wild-type in KRAS and BRAF. However, no difference in expression levels between KRAS- and BRAF-mutated tumors was evident for the miRNAs analyzed in this study.