RT Journal Article
SR Electronic
T1 Neoadjuvant Chemotherapy with Gemcitabine Plus Nab-paclitaxel Reduces the Number of Cancer-associated Fibroblasts Through Depletion of Pancreatic Stroma
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 337
OP 343
VO 38
IS 1
A1 TOMOHARU MIYASHITA
A1 HIDEHIRO TAJIMA
A1 ISAMU MAKINO
A1 MITSUYOSHI OKAZAKI
A1 TAKAHISA YAMAGUCHI
A1 YOSHINAO OHBATAKE
A1 SINICHI NAKANUMA
A1 HIRONORI HAYASHI
A1 HIROYUKI TAKAMURA
A1 ITASU NINOMIYA
A1 SACHIO FUSHIDA
A1 KOJI KISHIMOTO
A1 JOHN W. HARMON
A1 TETSUO OHTA
YR 2018
UL http://ar.iiarjournals.org/content/38/1/337.abstract
AB Background: In this study, the effects of neoadjuvant chemotherapy (NAC) on cancer-associated fibroblasts (CAFs) in pancreatic cancer stroma were investigated. Materials and Methods: Density of α-smooth muscle actin (αSMA)-positive fibroblasts in resected surgical specimens from untreated patients, patients receiving conventional gemcitabine plus S-1 (GS), and patients receiving gemcitabine plus nab-paclitaxel (GnP) was determined by hybrid cell counting. 18F-Fluorodeoxyglucose positron-emission tomography (FDG-PET) scans and carbohydrate antigen 19-9 (CA19-9) concentrations were used to assess tumor activity before and after chemotherapy in the GnP group. Results: In this retrospective study of 65 patients, αSMA expression was reduced in the GnP group, as revealed by markedly disorganized collagen and a low density of αSMA-positive fibroblasts. There were significantly fewer αSMA-positive fibroblasts in the GnP than in the untreated and GS groups, but there was no significant difference between the latter two groups. αSMA density reflected a decrease in standardized uptake value on FDG-PET, but not CA19-9 concentration, after GnP chemotherapy. Conclusion: These data suggest that the GnP regimen induces stromal depletion, resulting in fewer CAFs.