TY - JOUR T1 - The Long Noncoding RNA <em>OIP5-AS1</em> Is Involved in the Regulation of Cell Proliferation JF - Anticancer Research JO - Anticancer Res SP - 77 LP - 81 VL - 38 IS - 1 AU - MADOKA NAEMURA AU - MASAHIDE KUROKI AU - TOSHIYUKI TSUNODA AU - NAGISA ARIKAWA AU - YUUGA SAWATA AU - SENJI SHIRASAWA AU - YOJIRO KOTAKE Y1 - 2018/01/01 UR - http://ar.iiarjournals.org/content/38/1/77.abstract N2 - Background/Aim: OPA-interacting protein 5 antisense transcript 1 (OIP5-AS1) is a long noncoding RNA located on human chromosome 15q15.1 and transcribed in the opposite direction to OIP5. Here, we report that OIP5-AS1 is involved in regulating cell proliferation. Materials and Methods: HeLa cells were transfected with OIP5-AS1-targeting siRNA oligonucleotides and anti-sense oligonucleotides. The cells were harvested 72 h after transfection and subjected to quantitative reverse transcription-polymerase chain reaction (qRT-PCR), and cell-cycle and apoptosis analysis. Results: OIP5-AS1 was expressed at a lower level in cells harbouring an oncogenic kirsten rat sarcoma viral oncogene homolog (K-RAS) mutation than in cells expressing wild-type K-RAS. Silencing OIP5-AS1 with siRNA oligonucleotides or anti-sense oligonucleotides reduced HeLa cell proliferation. Apoptosis and cell-cycle analysis showed that silencing OIP5-AS1 did not cause apoptosis, but did cause G2/M phase cell-cycle arrest. Conclusion: These results suggest that OIP5-AS1 positively regulates cell proliferation by promoting G2/M phase progression. ER -