PT  - JOURNAL ARTICLE
AU  - CHIHIRO MATSUI
AU  - TOMOKA TAKATANI-NAKASE
AU  - SACHIE MAEDA
AU  - IKUHIKO NAKASE
AU  - KOICHI TAKAHASHI
TI  - Potential Roles of GLUT12 for Glucose Sensing and Cellular Migration in MCF-7 Human Breast Cancer Cells Under High Glucose Conditions
DP  - 2017 Dec 01
TA  - Anticancer Research
PG  - 6715--6722
VI  - 37
IP  - 12
4099  - http://ar.iiarjournals.org/content/37/12/6715.short
4100  - http://ar.iiarjournals.org/content/37/12/6715.full
SO  - Anticancer Res2017 Dec 01; 37
AB  - Background/Aim: Recent reports have indicated that hyperglycaemia is associated with breast cancer progression. High glucose conditions corresponding to hyperglycaemia significantly promote migration of MCF-7 human breast cancer cells, however, little is known about the mechanisms of glucose sensing for the acquisition of migratory properties by MCF-7 cells. This study investigated glucose sensing and mediation, which are responsible for the high motility of MCF-7 cells. Materials and Methods: We evaluated the migration of MCF-7 cells cultured in high glucose-containing medium and essential regulatory factors from the perspective of the glucose transport system. Results: We demonstrated that glucose transporter 12 (GLUT12) protein level increased in MCF-7 cells and co-localized with actin organization under high glucose conditions. Moreover, GLUT12-knockdown completely abrogated high glucose-induced migration, indicating that GLUT12 functionally participates in sensing high glucose concentrations. Conclusion: GLUT12 plays a critical role in the model of breast cancer progression through high glucose concentrations.