%0 Journal Article %A MUSTAPHA M. BOUHENNA %A BARBORA ORLIKOVA %A OUALID TALHI %A BEN SCHRAM %A DIANA C.G.A. PINTO %A NADIA TAIBI %A KHALDOUN BACHARI %A MARC DIEDERICH %A ARTUR M.S. SILVA %A NABIL MAMERI %T Anti-proliferative, Cytotoxic and NF-ĸB Inhibitory Properties of Spiro(Lactone-Cyclohexanone) Compounds in Human Leukemia %D 2017 %J Anticancer Research %P 5225-5233 %V 37 %N 9 %X Background/Aim: NF-ĸB affects most aspects of cellular physiology. Deregulation of NF-ĸB signaling is associated with inflammatory diseases and cancer. In this study, we evaluated the cytotoxic and NF-ĸB inhibition potential of new spiro(lactone-cyclohexanone) compounds in two different human leukemia cell lines (U937 and K562). Materials and Methods: The anti-proliferative effects of the spiro(lactone-cyclohexanone) compounds on human K562 and U937 cell lines was evaluated by trypan blue staining, as well as their involvement in NF-kB regulation were analyzed by luciferase reporter gene assay, Caspase-3/7 activities were evaluated to analyze apoptosis induction. Results: Both spiro(coumarin-cyclohexanone) 4 and spiro(6- methyllactone-cyclohexanone) 9 down-regulated cancer cell viability and proliferation. Compound 4 inhibited TNF-α-induced NF-ĸB activation in a dose-dependent manner and induced caspase-dependent apoptosis in both leukemia cell lines. Conclusion: Results show that compound 4 and compound 9 have potential as anti-cancer agents. In addition, compound 4 exerted NF-kB inhibition activity in leukemia cancer cells. %U https://ar.iiarjournals.org/content/anticanres/37/9/5225.full.pdf