PT - JOURNAL ARTICLE AU - ANCA MARIA CIMPEAN AU - VALERIA TARLUI AU - ALIN ADRIAN CUMPĂNAŞ AU - SORIN BOLINTINEANU AU - ANDREI CUMPĂNAŞ AU - MARIUS RAICA TI - Critical Overview of HER2 Assessement in Bladder Cancer: What Is Missing for a Better Therapeutic Approach? DP - 2017 Sep 01 TA - Anticancer Research PG - 4935--4942 VI - 37 IP - 9 4099 - http://ar.iiarjournals.org/content/37/9/4935.short 4100 - http://ar.iiarjournals.org/content/37/9/4935.full SO - Anticancer Res2017 Sep 01; 37 AB - Background/Aim: To assess the concordance between IHC and FISH and its influence on tumor type, grade and lympho-vascular invasion (LVI). Materials and Methods: HER2 immunohistochemistry (IHC) to 45 cases of bladder carcinoma, followed by fluorescent in situ hybridization (FISH) were applied. Results: 31.12% cases were IHC positive. Less than 35% of HER2-negative cases presented LVI and this percent increased to 54.54% for +1 HER2 cases. LVI increases up to 57.14% for +2 HER2 cases and slightly decreased for +3 HER2 cases to 42.85%. IHC/FISH concordance was found for 73.34% cases but 31.57% were previously negative and 36.36% scored as +1 by IHC had gene amplification as shown by FISH analysis. T3 was correlated with HER2-IHC (p=0.05) and HER2-FISH (p=0.01). Conclusion: Improved HER2 assessement is needed for urothelial carcinomas. HER2-IHC scored as 0-2 should be validated by and reclassified according to FISH analysis.