PT  - JOURNAL ARTICLE
AU  - YUTARO KOBAYASHI
AU  - HIROYUKI INAGAWA
AU  - CHIE KOHCHI
AU  - KATSUICHIRO OKAZAKI
AU  - RAN ZHANG
AU  - HIDEKI KOBARA
AU  - TSUTOMU MASAKI
AU  - GEN-ICHIRO SOMA
TI  - Lipopolysaccharides Derived from <em>Pantoea agglomerans</em> Can Promote the Phagocytic Activity of Amyloid β in Mouse Microglial Cells
DP  - 2017 Jul 01
TA  - Anticancer Research
PG  - 3917--3920
VI  - 37
IP  - 7
4099  - http://ar.iiarjournals.org/content/37/7/3917.short
4100  - http://ar.iiarjournals.org/content/37/7/3917.full
SO  - Anticancer Res2017 Jul 01; 37
AB  - Background/Aim: Recent studies reported that lipopolysaccharide (LPS) exhibits beneficial effects on prevention of immune-related diseases by activating macrophages. We previously demonstrated that pre-treatment with LPS derived from Pantoea agglomerans (LPSp) activated amyloid β (Aβ) phagocytosis in mouse primary microglia. In the present study, we further examined the promotory effect on phagocytosis of phagocytic particles in the C8-B4 microglia cell line. Materials and Methods: Phagocytic analysis of C8-B4 cells was evaluated using phagocytic particles (latex beads or HiLyte™ Fluor 488-conjugated Aβ1-42). Results: The phagocytic activity of latex beads was dependent on the concentration of beads and incubation time. LPSp, at as low as 100 pg/ml, significantly increased phagocytosis against the beads. In the experiment of Aβ1-42 phagocytosis, LPSp significantly increased Aβ phagocytic activity. Conclusion: LPSp treatment was confirmed to enhance Aβ1-42 phagocytosis by mouse microglia. It is suggested that the use of LPSp may be a potential promising candidate for the prevention of Alzheimer's disease.