@article {KOBAYASHI3917, author = {YUTARO KOBAYASHI and HIROYUKI INAGAWA and CHIE KOHCHI and KATSUICHIRO OKAZAKI and RAN ZHANG and HIDEKI KOBARA and TSUTOMU MASAKI and GEN-ICHIRO SOMA}, title = {Lipopolysaccharides Derived from Pantoea agglomerans Can Promote the Phagocytic Activity of Amyloid β in Mouse Microglial Cells}, volume = {37}, number = {7}, pages = {3917--3920}, year = {2017}, publisher = {International Institute of Anticancer Research}, abstract = {Background/Aim: Recent studies reported that lipopolysaccharide (LPS) exhibits beneficial effects on prevention of immune-related diseases by activating macrophages. We previously demonstrated that pre-treatment with LPS derived from Pantoea agglomerans (LPSp) activated amyloid β (Aβ) phagocytosis in mouse primary microglia. In the present study, we further examined the promotory effect on phagocytosis of phagocytic particles in the C8-B4 microglia cell line. Materials and Methods: Phagocytic analysis of C8-B4 cells was evaluated using phagocytic particles (latex beads or HiLyte{\texttrademark} Fluor 488-conjugated Aβ1-42). Results: The phagocytic activity of latex beads was dependent on the concentration of beads and incubation time. LPSp, at as low as 100 pg/ml, significantly increased phagocytosis against the beads. In the experiment of Aβ1-42 phagocytosis, LPSp significantly increased Aβ phagocytic activity. Conclusion: LPSp treatment was confirmed to enhance Aβ1-42 phagocytosis by mouse microglia. It is suggested that the use of LPSp may be a potential promising candidate for the prevention of Alzheimer{\textquoteright}s disease.}, issn = {0250-7005}, URL = {https://ar.iiarjournals.org/content/37/7/3917}, eprint = {https://ar.iiarjournals.org/content/37/7/3917.full.pdf}, journal = {Anticancer Research} }