RT Journal Article
SR Electronic
T1 Choline-Deficient-Diet-Induced Fatty Liver Is a Metastasis-Resistant Microenvironment
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 3429
OP 3434
VO 37
IS 7
A1 MIKI NAKAMURA
A1 ATSUSHI SUETSUGU
A1 KOSUKE HASEGAWA
A1 TAKURO MATSUMOTO
A1 HITOMI AOKI
A1 TAKAHIRO KUNISADA
A1 MASAHITO SHIMIZU
A1 SHIGETOYO SAJI
A1 HISATAKA MORIWAKI
A1 ROBERT M. HOFFMAN
YR 2017
UL http://ar.iiarjournals.org/content/37/7/3429.abstract
AB Background/Aim: Fatty liver disease is increasing in the developed and developing world. Liver metastasis from malignant lymphoma in the fatty liver is poorly understood. In a previous report, we developed color-coded imaging of the tumor microenvironment (TME) of the murine EL4-RFP malignant lymphoma during metastasis, including the lung. In the present report, we investigated the potential and microenvironment of the fatty liver induced by a choline-deficient diet as a metastatic site in this mouse lymphoma model. Materials and Methods: C57BL/6-GFP transgenic mice were fed with a choline-deficient diet in order to establish a fatty liver model. EL4-RFP cells were injected in the spleen of normal mice and fatty-liver mice. Metastases in mice with fatty liver or normal liver were imaged with the Olympus SZX7 microscope and the Olympus FV1000 confocal microscope. Results: Metastases of EL4-RFP were observed in the liver, ascites and bone marrow. Primary tumors were imaged in the spleen at the injection site. The fewest metastases were observed in the fatty liver. In addition, the fewest cancer-associated fibroblasts (CAFs) were observed in the fatty liver. Conclusion: The relative metastatic resistance of the fatty liver may be due to the reduced number of CAFs in the fatty livers. The mechanism of the effect of the choline-deficient diet is discussed.