RT Journal Article
SR Electronic
T1 Imaging the Role of Multinucleate Pancreatic Cancer Cells and Cancer-Associated Fibroblasts in Peritoneal Metastasis in Mouse Models
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 3435
OP 3440
VO 37
IS 7
A1 HASEGAWA, KOSUKE
A1 SUETSUGU, ATSUSHI
A1 NAKAMURA, MIKI
A1 MATSUMOTO, TAKURO
A1 AOKI, HITOMI
A1 KUNISADA, TAKAHIRO
A1 SHIMIZU, MASAHITO
A1 SAJI, SHIGETOYO
A1 MORIWAKI, HISATAKA
A1 HOFFMAN, ROBERT M.
YR 2017
UL http://ar.iiarjournals.org/content/37/7/3435.abstract
AB Background/Aim: The interaction between pancreatic-cancer cells and stromal cells in the tumor microenvironment (TME) is of particular importance in cancer progression and metastasis. The present report demonstrates the role of cancer-associated fibroblasts (CAFs) and multinucleate pancreatic-cancer cells in peritoneal metastasis. Materials and Methods: An orthotopic mouse model of pancreatic cancer was established with the human pancreatic cancer cell line BxPC3, which stably expresses green fluorescent protein (GFP). Results: BxPC3-GFP cells formed peritoneal metastases by week 18 after orthotopic implantation. Using an Olympus FV1000 confocal microscope, multi-nucleated cancer cells were frequently observed in the peritoneal metastases. The primary pancreatic tumor and peritoneal-metastases were harvested, cultured and then transplanted subcutaneously. Subcutaneous tumors established from peritoneal-metastatic cells were larger than subcutaneous tumors established from primary-tumor cells. Subcutaneous tumors of each type were subsequently cultured in vitro. CAFs were observed growing out from the tumors established from peritoneal-metastatic cells, but not the tumors established from the primary cancer. Conclusion: The results of the present study suggest that multi-nucleated cancer cells and CAFs were related to peritoneal metastasis of pancreatic cancer.