PT - JOURNAL ARTICLE AU - TITIKA-MARINA STRATI AU - VASSILIKI KOTOULA AU - IOANNIS KOSTOPOULOS AU - KYRIAKI MANOUSOU AU - CHRISTOS PAPADIMITRIOU AU - GEORGIOS LAZARIDIS AU - SOTIRIS LAKIS AU - GEORGE PENTHEROUDAKIS AU - DIMITRIOS PECTASIDES AU - ELISSAVET PAZARLI AU - CHRISTOS CHRISTODOULOU AU - EVANGELIA RAZIS AU - KITTY PAVLAKIS AU - CHRISTINA MAGKOU AU - SOFIA CHRISAFI AU - GERASIMOS ARAVANTINOS AU - DIMITRIOS BAFALOUKOS AU - PAVLOS PAPAKOSTAS AU - HELEN GOGAS AU - KONSTANTINE T. KALOGERAS AU - GEORGE FOUNTZILAS TI - Prognostic Subcellular Notch2, Notch3 and Jagged1 Localization Patterns in Early Triple-negative Breast Cancer DP - 2017 May 01 TA - Anticancer Research PG - 2323--2334 VI - 37 IP - 5 4099 - http://ar.iiarjournals.org/content/37/5/2323.short 4100 - http://ar.iiarjournals.org/content/37/5/2323.full SO - Anticancer Res2017 May 01; 37 AB - Background: The Notch pathway has been implicated in triple-negative breast cancer (TNBC). Herein, we studied the subcellular localization of the less investigated Notch2 and Notch3 and that of the Jagged1 (Jag1) ligand in patients with operable TNBC. Patients and Methods: We applied immunohistochemistry for Notch2, Notch3 and Jag1 in 333 tumors from TNBC patients treated with adjuvant anthracycline-based chemotherapy. We evaluated cytoplasmic (c), membranous (m) and nuclear (n) protein localization. Results: c-Notch2 (35% positive tumors), c-Notch3 (63%), c-Jag1 (43%), m-Notch3 (23%) and n-Jag1 (17%) were analyzed individually and by using hierarchical clustering for prognostic evaluation. Upon multivariate analysis, compared to high m-Notch3 in the absence of n-Jag1 (cluster 4), all other marker combinations (clusters 1, 2, 3) conferred significantly higher risk for relapse (p<0.05). Conclusion: Specific Notch3 and Jag1 subcellular localization patterns may provide clues for the behavior of the tumors and potentially for Jag1 targeting in TNBC patients.