PT  - JOURNAL ARTICLE
AU  - RUI M. GIL DA COSTA
AU  - RITA ARAÚJO
AU  - JOANA M.O. SANTOS
AU  - MARA FERNANDES
AU  - TIAGO NETO
AU  - HUGO SOUSA
AU  - JOANA RIBEIRO
AU  - MARGARIDA M.S.M. BASTOS
AU  - PAULA A. OLIVEIRA
AU  - DIOGO CARMO
AU  - FÁTIMA CASACA
AU  - SANDRA SILVA
AU  - CARLOS LOPES
AU  - RUI MEDEIROS
TI  - Regulation of miRNA-146a and miRNA-150 Levels by Celecoxib in Premalignant Lesions of K14-HPV16 Mice
DP  - 2017 Jun 01
TA  - Anticancer Research
PG  - 2913--2918
VI  - 37
IP  - 6
4099  - http://ar.iiarjournals.org/content/37/6/2913.short
4100  - http://ar.iiarjournals.org/content/37/6/2913.full
SO  - Anticancer Res2017 Jun 01; 37
AB  - Background/Aim: Human papillomavirus type 16 (HPV16) induces various types of cancer in several locations. Microenvironmental microRNAs (miRNAs) such as miRNA-146a and miRNA-150 regulate cancer-associated inflammation and are involved in HPV-induced carcinogenesis. We studied the effects of celecoxib on the expression of these two miRNAs in HPV16-induced lesions. Materials and Methods: Female transgenic (HPV16+/−) and wild-type (HPV16−/−) mice were administered 75 mg/kg/day celecoxib orally (treatment groups) or placebo (control groups) for four weeks. Skin samples were classified histologically, or used for miRNA analysis by quantitative real-time PCR. Results: HPV16+/− mice showed higher miRNA-146a and miRNA-150 expression levels compared to wild-type animals. Celecoxib further increased miRNA-150 (p<0.05) and miRNA-146a levels in treated animals. Celecoxib-treated HPV16+/− animals also showed reduced incidence of epidermal dysplasia and reduced inflammation, compared to untreated mice. Conclusion: In this model, celecoxib may be able to regulate tumour-associated inflammation, through mechanisms involving the regulation of miRNA-146a and miRNA-150.