RT Journal Article
SR Electronic
T1 3,4’,7-O-trimethylquercetin Inhibits Invasion and Migration of Ovarian Cancer Cells
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 2823
OP 2829
VO 37
IS 6
A1 KOSEI YAMAUCHI
A1 SYEDA H. AFROZE
A1 TOHRU MITSUNAGA
A1 TIMOTHY C. MCCORMICK
A1 THOMAS J. KUEHL
A1 DAVID C. ZAWIEJA
A1 MOHAMMAD N. UDDIN
YR 2017
UL http://ar.iiarjournals.org/content/37/6/2823.abstract
AB Background/Aim: Methylquercetin, 3,4’,7-O-trimethylquercetin (34’7TMQ), has been reported to inhibit metastasis. Recently, we demonstrated that 34’7TMQ inhibited the in vitro melanoma B16 cell metastatic activity. We evaluated the effect of 34’7TMQ on three ovarian cancer cells (SK-OV-3, CRL11731 and CRL1978). Materials and Methods: Proliferation, migration and invasion were measured in 34’7TMQ-treated ovarian cancer cells by commercially available kits. We also evaluated the expression of proliferating cell nuclear antigen (PCNA), urokinase plasminogen activator (uPA), plasminogen activator inhibitor 1 (PAI-1) and matrix metalloproteinase (MMP)-2 by western blot analysis. Results: 34’7TMQ inhibited ovarian cancer cell migration and invasion without effecting proliferation. Furthermore, 34’7TMQ inhibited the expression of uPA and MMP-2; however, it had no effect on PAI-1 and PCNA. Conclusion: 34’7TMQ significantly regulates the expressions of protein to inhibit metastasis in ovarian cancers, while the regulatory effects of 34’7TMQ vary between different ovarian cancer cell lines.