RT Journal Article
SR Electronic
T1 Alpha-bisabolol Promotes Glioma Cell Death by Modulating the Adenosinergic System
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 1819
OP 1823
VO 37
IS 4
A1 FRANCIANE BRACKMANN MENDES
A1 LETÍCIA SCUSSEL BERGAMIN
A1 CRISTIANE DOS SANTOS STUEPP
A1 ELIZANDRA BRAGANHOL
A1 THATIANA TERROSO
A1 ADRIANA RAFIN POHLMANN
A1 SÍLVIA STANISÇUASKI GUTERRES
A1 ANA MARIA OLIVEIRA BATTASTINI
YR 2017
UL http://ar.iiarjournals.org/content/37/4/1819.abstract
AB Background/Aim: Glioblastoma multiforme is the most malignant type of glioma. Alpha-bisabolol is an essential oil reported as a potent cell death agent. In the present work, we evaluated the effect of alpha-bisabolol on ecto-5’-nucleotidase/CD73, the most well-characterized enzymatic source of adenosine, present in lipid rafts. Materials and Methods: Glioma cells were treated with alpha-bisabolol and, in some experiments, pre-treated with an A3 antagonist. MTT assay (viability), malachite green method (ecto-5’-nucleotidase/CD73 activity) and quantitative polymerase chain reaction (qPCR) (A3 mRNA) were carried out. Results: Alpha-bisabolol led to a decrease in C6 and U138-MG glioma cells viability, accompanied by an increase in ecto-5’-NT/CD73 activity. Pre-treatment with an A3 antagonist reverted the effect of α-bisabolol with an increase of mRNA expression of this receptor. Conclusion: Our data indicated the participation of ecto-5’-nucleotidase/CD73 and A3 receptor in the anti-proliferative effect of α-bisabolol on glioma cells.