@article {P1637, author = {NIPIN S P and DONG YOUNG KANG and BAEK JOONG KIM and YOUN HEE JOUNG and PRAMOD DARVIN and HYO JOO BYUN and JOONG GON KIM and JE UK PARK and YOUNG MOK YANG}, title = {Methylsulfonylmethane Induces G1 Arrest and Mitochondrial Apoptosis in YD-38 Gingival Cancer Cells}, volume = {37}, number = {4}, pages = {1637--1646}, year = {2017}, publisher = {International Institute of Anticancer Research}, abstract = {Gingival squamous cell carcinoma is a rare form of cancer that accounts for less than 10\% of all head and neck cancers. Targeted therapies with natural compounds are of interest because they possess high efficacy with fewer side-effects. Methylsulfonylmethane (MSM) is an organic sulfur-containing compound with anticancer activities. The main goal of this study was to induce proliferation inhibition and apoptosis in the metastatic YD-38 cell line. MSM up-regulated expression of P21Waf1/Cip1 and P27Kip1 genes and down-regulated expression of cyclin D1 (CCND1) and CDK4. Moreover, treatment with MSM induced apoptosis and up-regulation of BAX in YD-38 cells. In accordance, the expression of the BCL-2 and BCL-XL, were inhibited, indicating the role of mitochondria in MSM-induced apoptosis. Analysis of mitochondrial integrity showed a loss of mitochondrial potential with an increased level of cytochrome c in the cytosol compared to mitochondria. Active CASPASE-3 (CASP3) was also observed, confirming that MSM-induced apoptosis is caspase-mediated.}, issn = {0250-7005}, URL = {https://ar.iiarjournals.org/content/37/4/1637}, eprint = {https://ar.iiarjournals.org/content/37/4/1637.full.pdf}, journal = {Anticancer Research} }