RT Journal Article
SR Electronic
T1 Correlation of Blood T-Cells to Intratumoural Density and Location of CD3<sup>+</sup> and CD8<sup>+</sup> T-Cells in Colorectal Cancer
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 675
OP 683
VO 37
IS 2
A1 HANNE R. HAGLAND
A1 DORDI LEA
A1 MARTIN M. WATSON
A1 KJETIL SØREIDE
YR 2017
UL http://ar.iiarjournals.org/content/37/2/675.abstract
AB Aim: To test the feasibility of conducting parallel analyses of circulating T-cells in blood and intratumoural T-cells in colorectal cancer. A pre-operative ‘liquid biopsy’ to determine immune status would facilitate clinical decision-making. Materials and Methods: A total of 18 patients with stage I-III colorectal cancer (CRC) were included. Blood was analyzed for T-cell type (CD3+, CD4+ and CD8+) and count using flow cytometry. Intratumoural T-cells were stained using immunohistochemistry and quantified by digital pathology. Tumour location was defined as invasive front (IF) or tumour center (TC). Results: The number of CD3+ and CD4+ T-cells in pre-surgical blood samples correlated with the number of CD3+ T-cells found in the IF (Spearman ϱ=0.558, p&lt;0.05 and 0.598, p&lt;0.01 respectively) and CD3+ in the TC (ϱ=0.496, p&lt;0.05, and ϱ=0.637, p&lt;0.01, respectively). A strong correlation was found between CD4+ cells in blood and CD8+ T-cells found in the TC and IF (ϱ=0.602 and ϱ=0.591, p&lt;0.01). Conclusion: There is a correlation between blood CD3+ and CD4+ T-cells and the T-cells found at the TC and IF.