TY - JOUR T1 - Long Noncoding RNA <em>PANDA</em> Positively Regulates Proliferation of Osteosarcoma Cells JF - Anticancer Research JO - Anticancer Res SP - 81 LP - 85 VL - 37 IS - 1 AU - YOJIRO KOTAKE AU - TAIKI GOTO AU - MADOKA NAEMURA AU - YASUTOSHI INOUE AU - HARUNA OKAMOTO AU - KEIICHIRO TAHARA Y1 - 2017/01/01 UR - http://ar.iiarjournals.org/content/37/1/81.abstract N2 - Background: A long noncoding RNA, p21-associated ncRNA DNA damage-activated (PANDA), associates with nuclear transcription factor Y subunit alpha (NF-YA) and inhibits its binding to promoters of apoptosis-related genes, thereby repressing apoptosis in normal human fibroblasts. Here, we show that PANDA is involved in regulating proliferation in the U2OS human osteosarcoma cell line. Materials and Methods: U2OS cells were transfected with siRNAs against PANDA 72 h later and they were subjected to reverse transcription-polymerase chain reaction (RT-PCR), quantitative RT-PCR and cell-cycle analysis. Results: PANDA was highly expressed in U2OS cells, and its expression was induced by DNA damage. Silencing PANDA caused arrest at the G1 phase of the cell cycle, leading to inhibition of cell proliferation. Quantitative RT-PCR showed that silencing PANDA increased mRNA levels of the cyclin-dependent kinase inhibitor p18, which caused G1 phase arrest. Conclusion: These results suggest that PANDA promotes G1-S transition by repressing p18 transcription, and thus promotes U2OS cell proliferation. ER -