RT Journal Article
SR Electronic
T1 Overexpression of PBK/TOPK Contributes to Tumor Development and Poor Outcome of Esophageal Squamous Cell Carcinoma
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 6457
OP 6466
VO 36
IS 12
A1 TAKUMA OHASHI
A1 SHUHEI KOMATSU
A1 DAISUKE ICHIKAWA
A1 MAHITO MIYAMAE
A1 WATARU OKAJIMA
A1 TAISUKE IMAMURA
A1 JUN KIUCHI
A1 KEIJI NISHIBEPPU
A1 TOSHIYUKI KOSUGA
A1 HIROTAKA KONISHI
A1 ATSUSHI SHIOZAKI
A1 HITOSHI FUJIWARA
A1 KAZUMA OKAMOTO
A1 HITOSHI TSUDA
A1 EIGO OTSUJI
YR 2016
UL http://ar.iiarjournals.org/content/36/12/6457.abstract
AB Background: PDZ-binding kinase/T-cell-originated protein kinase (PBK/TOPK) is a serine-threonine kinase and overexpressed in various types of cancer. PBK/TOPK is associated with tumor cell development and progression through suppression of p53 function. In this study, we tested whether PBK acts as a cancer-promoting factor by being overexpressed in esophageal squamous cell carcinoma (ESCC). Materials and Methods: We analyzed PBK/TOPK expression in 15 ESCC cell lines, and 54 primary ESCC tumors that were curatively resected between 1994 and 2007. Results: Overexpression of the PBK/TOPK protein was detected in 93% (14/15) ESCC cell lines and 19% (10/54) primary ESCC tumor samples, and significantly correlated with macroscopic appearance and tumor depth. PBK/TOPK positivity was independently associated with worse outcome in multivariate analysis (p=0.0235, hazard ratio=3.58). Knockdown of PBK/TOPK using specific siRNAs inhibited the cell proliferation, invasion/migration of PBK/TOPK-overexpressing ESCC cell lines. Conclusion: These findings suggest that PBK/TOPK plays a crucial role in tumor malignant potential through its overexpression in ESCC.